Playful E-textile Sonic Interaction for Socially Engaged and Open-Ended Play Between Autistic Children

Research on the potential benefits of technology for autistic children is an emergent field in Human-Computer Interaction (HCI), especially within the Child-Computer Interaction Community. This thesis contributes a design approach grounded in theories of play, cognitive development, and autism to expand the discourse on methodological guidelines for performing empirical studies with non-verbal autistic children and to extend the design space to cater to the socio-emotional and sensory needs of this population. The thesis reveals how sonic e-textile Tangible User Interfaces (TUIs) can be used effectively to mediate children’s social participation in playful activities. This is demonstrated through developing three explorative field-studies conducted at a specialist school based in North-East London where two sonic e-textile playful TUIs, namely Mazi and Olly, have been created and tested with three groups of autistic children aged between 5-10. The three studies ran over the period of three years and were designed to investigate the potentials of TUIs as shareable toys during leisure and recreational activities to a) support social and playful interactions among peers and b) provide opportunities for self-regulation. The key contributions of this thesis are the designs of two tangible user interfaces, which offer a set of design approaches to guide researchers through creating shareable and playful tangibles for non-verbal autistic children; a framework for analysis and a thorough evaluation process that other researchers could use to assess the efficacy of playful TUI designs for nonverbal autistic children; and an in-depth discussion about the research process, which offers a new perspective about holistic designs and evaluation of technologies that aim to scaffold play in groups non-verbal autistic children

Modern public internal control systems and accountability in health care organizations

The introduction of NPM principles changed the meaning and the contents of public sector accountability as it is no longer intended as a mere compliance with rules and procedures but as the need to give an account to the citizenry, in terms of how results are achieved and resources are used in their pursuit. Accountability for results requires the modernisation of traditional control systems. These check whether regulations are followed without ensuring that the achievement of objectives is controlled. Confirming the growing need in modernizing internal control systems and increasing managerial accountability and transparency in spending public money, the European Commission has developed a reference model for the public sector: Public Internal Financial Control – PIfC. The paper tries to contribute the relevant debate on the usefulness of internal control systems by exploring the implicit positive relationship between them and managerial accountability envisaged in the PIfC model. To this end the research focuses on a case study set in the context of Italian health care sector and in the main health care organization located in the Region of Sardinia

Mazi: a Tangible Toy for Collaborative Play between Children with Autism

Best Demo Awar

Indicators of biodiversity in an intensively cultivated and heavily human modified landscape

Nowadays, the loss of biodiversity in agroecosystems due to the intensification of farming practices is happening very fast, and therefore, stopping or slowing it down should be a priority for conservation. To detect changes in these environmental contexts, one approach contemplates focusing on a limited set of indicator species that can alert us to ongoing changes in progress. In this research, we aimed to measure the biodiversity of vertebrates using a multi-taxa approach in an intensively cultivated and highly inhabited area located in northern Italy. We investigated the relationships between biodiversity and environmental characteristics and we identified the taxonomic groups that can be used as indicators of biodiversity. Data collection was carried out in 2016 with different methods depending on the taxonomic group, in 131 sampling units chosen using a Tessellation Stratified Sampling. Then we calculated for each sampling unit a standardized Biodiversity Index, which was related to environmental variables concerning the land use and the landscape configuration using Multiple Linear Regression Analysis and Information-Theoretic approach. We used correlation analyses and the Indicator Species Analysis (IndVal) to identify the taxonomic groups and species that can be used as indicators of biodiversity. Biodiversity was positively related to the number of patches of natural vegetation, whereas it was negatively affected by the number of patches of artificial surfaces and by habitat diversity. Our findings agree with those obtained by many other researchers, which pointed out that agroecosystems provide adequate shelters, suitable foraging habitats and nesting sites. The negative effect of habitat diversity was explained by the area-heterogeneity trade-off. Therefore, sites with high heterogeneity will not contain enough cover of residual natural vegetation, essential to maintain high biodiversity, because increasing compositional heterogeneity within a fixed area simultaneously reduces the surface of each cover type. The analyses showed that birds and reptiles might be used as biodiversity indicators of vertebrates. Eurasian Magpie and Green Whip Snake, both generalist species, were associated with sites of low biodiversity, whereas seven birds, both generalists and farmland specialists, were associated with sites of medium biodiversity. In high biodiversity sites there were not indicator species. To conclude, in less natural environments, such as urban and agricultural landscapes, a combination of specialist and generalist indicator species seems adequate to monitor biodiversity changes. Our findings increase the knowledge of these very dynamic ecosystems, being important both to plan strategies for biodiversity conservation and to guarantee ecosystems services useful for humans

Efficiency of DNA mini-barcoding to assess mislabeling in commercial fish products in italy: An overview of the last decade

The problem of fish traceability in processed products is still an important issue in food safety. Major attention is nowadays dedicated to consumer health and prevention of possible frauds regulated by national and international laws. For this reason, a technical approach is fun-damental in revealing mislabeling at different levels. In particular, the use of genetic markers has been standardized and DNA barcoding is considered the gold-standard strategy to examine and prevent species substitution. Considering the richness of available DNA databases, it is nowadays possible to rapidly reach a reliable taxonomy at the species level. Among different approaches, an innovative method based on DNA mini barcoding has recently been proposed at an international level. Starting from this evidence, we herein illustrate an investigation dealing with the evolution of this topic in Italy over the last decade. The molecular analysis of 71 commercial fish samples based on mini-COI sequencing with two different primer sets reached an amplification success rate of 87.3 and 97.2%. The investigation revealed four major frauds (5.8%) and four minor ones (5.8%). Results highlighted a decrease in incorrect labeling in Italy from 32% to 11.6% over the last decade, although a recurrent involvement of “endangered” species sensu IUCN was still observed

Ovothiol ensures the correct developmental programme of the sea urchin Paracentrotus lividus embryo

Ovothiols are π-methyl-5-thiohistidines produced in great amounts in sea urchin eggs, where they can act as protective agents against the oxidative burst at fertilization and environmental stressors during development. Here we examined the biological relevance of ovothiol during the embryogenesis of the sea urchin Paracentrotus lividus by assessing the localization of the key biosynthetic enzyme OvoA, both at transcript and protein level, and perturbing its protein translation by morpholino antisense oligonucleotide-mediated knockdown experiments. In addition, we explored the possible involvement of ovothiol in the inflammatory response by assessing ovoA gene expression and protein localization following exposure to bacterial lipopolysaccharide. The results of the present study suggest that ovothiol may be a key regulator of cell proliferation in early developing embryos. Moreover, the localization of OvoA in key larval cells and tissues, in control and inflammatory conditions, suggests that ovothiol may ensure larval skeleton formation and mediate inflammatory processes triggered by bacterial infection. This work significantly contributes to the understanding of the biological function of ovothiols in marine organisms, and may provide new inspiration for the identification of the biological activities of ovothiols in humans, considering the pharmacological potential of these molecules

Strengths and weaknesses of different italian fish indices under the water framework directive guidelines

The ISECI (or F index) has been the first fish index to be recommended by the Italian Ministry of the Environment to assess the rivers ecological status with regard to fish communities, in accordance with the Water Framework Directive 2000/60 EC. In addition to ISECI, other fish indices have been developed such as the Forneris Ichthyic Index (I.I.) and a revised version of ISECI, the so-called NISECI. The latter is nowadays the reference Italian index in the framework of the Water Framework Directive. In this work, we analyzed 30 sampling sites along 18 watercourses in Northern Italy and computed the results of fish monitoring to evaluate the strength of ISECI and NISECI, as well as to assess weak points limiting their application. We detected several issues that undermine the ISECI effectiveness. The weakest point regarded the mismatch between the expected reference fish community and the sampled ones, which decreased the overall algorithm efficiency in the evaluation process. On the other hand, the results confirm the improvements introduced by NISECI. Although with some advancement, all three proposed indices revealed their weaknesses in the overall assessment of the ecological status of the water course, as also highlighted by a pioneering comparison with three expert-based blind judgements

Proteomic Analysis Reveals a Mitochondrial Remodeling of βTC3 Cells in Response to Nanotopography

Recently, using cluster-assembled zirconia substrates with tailored roughness produced by supersonic cluster beam deposition, we demonstrated that \u3b2 cells can sense nanoscale features of the substrate and can translate these stimuli into a mechanotransductive pathway capable of preserveing \u3b2-cell differentiation and function in vitro in long-term cultures of human islets. Using the same proteomic approach, we now focused on the mitochondrial fraction of \u3b2TC3 cells grown on the same zirconia substrates and characterized the morphological and proteomic modifications induced by the nanostructure. The results suggest that, in \u3b2TC3 cells, mitochondria are perturbed by the nanotopography and activate a program involving metabolism modification and modulation of their interplay with other organelles. Data were confirmed in INS1E, a different \u3b2-cell model. The change induced by the nanostructure can be pro-survival and prime mitochondria for a metabolic switch to match the new cell needs

The genetics of adaptation in freshwater Eurasian shad (Alosa)

Studying the genetics of phenotypic convergence can yield important insights into adaptive evolution. Here, we conducted a comparative genomic study of four lineages (species and subspecies) of anadromous shad (Alosa) that have independently evolved life cycles entirely completed in freshwater. Three naturally diverged (A. fallax lacustris, A. f. killarnensis, and A. macedonica), and the fourth (A. alosa) was artificially landlocked during the last century. To conduct this analysis, we assembled and annotated a draft of the A. alosa genome and generated whole-genome sequencing for 16 anadromous and freshwater populations of shad. Widespread evidence for parallel genetic changes in freshwater populations within lineages was found. In freshwater A. alosa, which have only been diverging for tens of generations, this shows that parallel adaptive evolution can rapidly occur. However, parallel genetic changes across lineages were comparatively rare. The degree of genetic parallelism was not strongly related to the number of shared polymorphisms between lineages, thus suggesting that other factors such as divergence among ancestral populations or environmental variation may influence genetic parallelism across these lineages. These overall patterns were exemplified by genetic differentiation involving a paralog of ATPase-α1 that appears to be under selection in just two of the more distantly related lineages studied, A. f. lacustris and A. alosa. Our findings provide insights into the genetic architecture of adaptation and parallel evolution along a continuum of population divergence

Cortisol, Temperament and Serotonin in Karate Combats: An Evolutionary Psychobiological Perspective

Objectives: There is evidence suggesting that in martial arts competitions athletes characterized by higher anxiety and harm avoidance may be more likely to lose a fight. This psychological profile has been hypothesized to explain in part the observation that cortisol is higher in losers before and in response to a competition. An important research target that needs further exploration is the identification of phenotypic traits that can be helpful in predicting athletes’ performance. Here we present a brief description of the theoretical bases that drives our research in the evolutionary psychobiology of sports and illustrate preliminary data on the relationship between the 5HTTLPR genotype, salivary cortisol, temperament and competition. Methods: Sixty-five healthy male non-professional athletes provided saliva samples 10 min before and after a kumite session and filled out the Tridimensional Personality Questionnaire. Results: Salivary cortisol levels 10 min before the competition were higher in losers and in athletes with the S allele. Temperament was associated with competition outcome and cortisol: losers were characterized by higher scores of harm avoidance and harm avoidance was positively correlated with cortisol levels. Conclusions: The results confirm previous findings linking temperamental traits, pre-and post- competition physiological stress response with competition outcome in kumite fight. Moreover, they indicate an association between the 5HTTLPR polymorphism and pre-competition salivary cortisol, thus providing a preliminary but non-conclusive evidence on the role played by the 5HTTLPR genotype as a vulnerability factor in sport competition